Automated culture system is developed for long-term culture and differentiation of human induced pluripotent stem cells (hiSPCs). It is designed for in-vivo mimicking of nutrient feeding in a perfusion-diffusion regime. It allows reducing time and laborious efforts as well as risks of contamination. Typically, it is composed of different separate units to control cell culture medium with selected factors injection and distribution. The system could also be placed in an incubator. Depending on applications, different configurations could be realized. The whole processes can then be programmed with minimum human intervention with good cell culture reproducibility. It can also be combined with other fluidic element, allowing automated cell sorting, labeling, sample preparing, etc. The system is applicable to both dish , organ chip based assays developed by MesoBioTech.
AC-121 is an integrated system allowing auto feeding and recycling with one culture medium to one dish.
AC-121 is an integrated system allowing auto feeding and recycling with one culture medium up to six dish.
AC-621 is an integrated system allowing auto distribution up to 6 factors to one culture dish.
Configurations& parameters | Peristaltic pump: 0-300rmp/min |
Rotary valve: 8 channels, response time 1s | |
Flow speed: 3ul/min-40ml/min | |
Interface communication: RS485 | |
General parameters | Gas fittings: Luer |
Dimensions: 155 × 175 × 100 mm | |
Weight: 1Kg |
Immunofluorescence images of mature neurons after differentiation for 78 days by manual and automated method
Microscopy images of mature cardiac tissue sheets after differentiation for 20 days by advanced cell culture system